Microbial diversity in Tunisian olive fermentation brine as evaluated by small subunit rRNA - Single strand conformation polymorphism analysis.
Identifieur interne : 000050 ( Main/Exploration ); précédent : 000049; suivant : 000051Microbial diversity in Tunisian olive fermentation brine as evaluated by small subunit rRNA - Single strand conformation polymorphism analysis.
Auteurs : Mohamed Chamkha [Tunisie] ; Sami Sayadi ; Valérie Bru ; Jean-Jacques GodonSource :
- International journal of food microbiology [ 0168-1605 ] ; 2008.
Descripteurs français
- KwdFr :
- Fermentation (MeSH), Gammaproteobacteria (isolement et purification), Lactobacillus (isolement et purification), Microbiologie alimentaire (MeSH), Olea (microbiologie), Petite sous-unité du ribosome (génétique), Pichia (isolement et purification), Polymorphisme de conformation simple brin (MeSH), Réaction de polymérisation en chaîne (méthodes), Tunisie (MeSH).
- MESH :
- génétique : Petite sous-unité du ribosome.
- isolement et purification : Gammaproteobacteria, Lactobacillus, Pichia.
- microbiologie : Olea.
- méthodes : Réaction de polymérisation en chaîne.
- Fermentation, Microbiologie alimentaire, Polymorphisme de conformation simple brin, Tunisie.
- Wicri :
- geographic : Tunisie.
English descriptors
- KwdEn :
- Fermentation (MeSH), Food Microbiology (MeSH), Gammaproteobacteria (isolation & purification), Lactobacillus (isolation & purification), Olea (microbiology), Pichia (isolation & purification), Polymerase Chain Reaction (methods), Polymorphism, Single-Stranded Conformational (MeSH), Ribosome Subunits, Small (genetics), Tunisia (MeSH).
- MESH :
- geographic : Tunisia.
- genetics : Ribosome Subunits, Small.
- isolation & purification : Gammaproteobacteria, Lactobacillus, Pichia.
- methods : Polymerase Chain Reaction.
- microbiology : Olea.
- Fermentation, Food Microbiology, Polymorphism, Single-Stranded Conformational.
Abstract
The microbial diversity of a Tunisian olive fermentation brine was analysed using a culture-independent approach based on the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). SSCP patterns show a remarkably simple microbial community but higher for bacterial community than for the eukaryotic community. This study did not show the presence of archaeal populations. After PCR amplification, two small subunit (SSU) rRNA clone libraries of Bacteria and Eucarya populations were established. Three bacteria and only one eukaryotic phylotype were identified. Two dominant bacteria showed 100% phylogenetic similarity to the 16S rRNA sequences of Lactobacillus plantarum and Lactobacillus collinoides and represent 85% of bacterial community. The third bacteria phylotype was phylogenetically close related to the 16S rRNA sequence of a moderately halophilic bacterium belonging to the class gamma Proteobacteria. The dominant eukaryotic phylotype was identified as a Pichia membranaefaciens.
DOI: 10.1016/j.ijfoodmicro.2007.11.052
PubMed: 18191493
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000775
- to stream PubMed, to step Curation: 000771
- to stream PubMed, to step Checkpoint: 000767
- to stream Main, to step Merge: 000050
- to stream Main, to step Curation: 000050
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Microbial diversity in Tunisian olive fermentation brine as evaluated by small subunit rRNA - Single strand conformation polymorphism analysis.</title><author><name sortKey="Chamkha, Mohamed" sort="Chamkha, Mohamed" uniqKey="Chamkha M" first="Mohamed" last="Chamkha">Mohamed Chamkha</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire des Bioprocédés, Centre de Biotechnologie de Sfax, BP K , 3038 Sfax, Tunisia. mohamed.chamkha@cbs.rnrt.tn</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratoire des Bioprocédés, Centre de Biotechnologie de Sfax, BP K , 3038 Sfax</wicri:regionArea><wicri:noRegion>3038 Sfax</wicri:noRegion></affiliation></author><author><name sortKey="Sayadi, Sami" sort="Sayadi, Sami" uniqKey="Sayadi S" first="Sami" last="Sayadi">Sami Sayadi</name></author><author><name sortKey="Bru, Valerie" sort="Bru, Valerie" uniqKey="Bru V" first="Valérie" last="Bru">Valérie Bru</name></author><author><name sortKey="Godon, Jean Jacques" sort="Godon, Jean Jacques" uniqKey="Godon J" first="Jean-Jacques" last="Godon">Jean-Jacques Godon</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:18191493</idno><idno type="pmid">18191493</idno><idno type="doi">10.1016/j.ijfoodmicro.2007.11.052</idno><idno type="wicri:Area/PubMed/Corpus">000775</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000775</idno><idno type="wicri:Area/PubMed/Curation">000771</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000771</idno><idno type="wicri:Area/PubMed/Checkpoint">000767</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000767</idno><idno type="wicri:Area/Main/Merge">000050</idno><idno type="wicri:Area/Main/Curation">000050</idno><idno type="wicri:Area/Main/Exploration">000050</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Microbial diversity in Tunisian olive fermentation brine as evaluated by small subunit rRNA - Single strand conformation polymorphism analysis.</title><author><name sortKey="Chamkha, Mohamed" sort="Chamkha, Mohamed" uniqKey="Chamkha M" first="Mohamed" last="Chamkha">Mohamed Chamkha</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire des Bioprocédés, Centre de Biotechnologie de Sfax, BP K , 3038 Sfax, Tunisia. mohamed.chamkha@cbs.rnrt.tn</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratoire des Bioprocédés, Centre de Biotechnologie de Sfax, BP K , 3038 Sfax</wicri:regionArea><wicri:noRegion>3038 Sfax</wicri:noRegion></affiliation></author><author><name sortKey="Sayadi, Sami" sort="Sayadi, Sami" uniqKey="Sayadi S" first="Sami" last="Sayadi">Sami Sayadi</name></author><author><name sortKey="Bru, Valerie" sort="Bru, Valerie" uniqKey="Bru V" first="Valérie" last="Bru">Valérie Bru</name></author><author><name sortKey="Godon, Jean Jacques" sort="Godon, Jean Jacques" uniqKey="Godon J" first="Jean-Jacques" last="Godon">Jean-Jacques Godon</name></author></analytic><series><title level="j">International journal of food microbiology</title><idno type="ISSN">0168-1605</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Fermentation (MeSH)</term><term>Food Microbiology (MeSH)</term><term>Gammaproteobacteria (isolation & purification)</term><term>Lactobacillus (isolation & purification)</term><term>Olea (microbiology)</term><term>Pichia (isolation & purification)</term><term>Polymerase Chain Reaction (methods)</term><term>Polymorphism, Single-Stranded Conformational (MeSH)</term><term>Ribosome Subunits, Small (genetics)</term><term>Tunisia (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Fermentation (MeSH)</term><term>Gammaproteobacteria (isolement et purification)</term><term>Lactobacillus (isolement et purification)</term><term>Microbiologie alimentaire (MeSH)</term><term>Olea (microbiologie)</term><term>Petite sous-unité du ribosome (génétique)</term><term>Pichia (isolement et purification)</term><term>Polymorphisme de conformation simple brin (MeSH)</term><term>Réaction de polymérisation en chaîne (méthodes)</term><term>Tunisie (MeSH)</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Tunisia</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Ribosome Subunits, Small</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Petite sous-unité du ribosome</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Gammaproteobacteria</term><term>Lactobacillus</term><term>Pichia</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Gammaproteobacteria</term><term>Lactobacillus</term><term>Pichia</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Olea</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Olea</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Réaction de polymérisation en chaîne</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Fermentation</term><term>Food Microbiology</term><term>Polymorphism, Single-Stranded Conformational</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Fermentation</term><term>Microbiologie alimentaire</term><term>Polymorphisme de conformation simple brin</term><term>Tunisie</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Tunisie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The microbial diversity of a Tunisian olive fermentation brine was analysed using a culture-independent approach based on the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). SSCP patterns show a remarkably simple microbial community but higher for bacterial community than for the eukaryotic community. This study did not show the presence of archaeal populations. After PCR amplification, two small subunit (SSU) rRNA clone libraries of Bacteria and Eucarya populations were established. Three bacteria and only one eukaryotic phylotype were identified. Two dominant bacteria showed 100% phylogenetic similarity to the 16S rRNA sequences of Lactobacillus plantarum and Lactobacillus collinoides and represent 85% of bacterial community. The third bacteria phylotype was phylogenetically close related to the 16S rRNA sequence of a moderately halophilic bacterium belonging to the class gamma Proteobacteria. The dominant eukaryotic phylotype was identified as a Pichia membranaefaciens.</div></front></TEI><affiliations><list><country><li>Tunisie</li></country></list><tree><noCountry><name sortKey="Bru, Valerie" sort="Bru, Valerie" uniqKey="Bru V" first="Valérie" last="Bru">Valérie Bru</name><name sortKey="Godon, Jean Jacques" sort="Godon, Jean Jacques" uniqKey="Godon J" first="Jean-Jacques" last="Godon">Jean-Jacques Godon</name><name sortKey="Sayadi, Sami" sort="Sayadi, Sami" uniqKey="Sayadi S" first="Sami" last="Sayadi">Sami Sayadi</name></noCountry><country name="Tunisie"><noRegion><name sortKey="Chamkha, Mohamed" sort="Chamkha, Mohamed" uniqKey="Chamkha M" first="Mohamed" last="Chamkha">Mohamed Chamkha</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/MaghrebDataLibMedV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000050 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000050 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= MaghrebDataLibMedV2
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:18191493
|texte= Microbial diversity in Tunisian olive fermentation brine as evaluated by small subunit rRNA - Single strand conformation polymorphism analysis.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:18191493" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a MaghrebDataLibMedV2
| This area was generated with Dilib version V0.6.38. |  |